A series of reagents will be used to separate the dna from the cells. List 2 organelles that were not visible but should have been in the cheek cell. Learn how to extract dna from cells and describe the. Add one drop of methylene blue onto the water and cell solution to stain the cheek cells for observation. The cells seen are squamous epithelial cells from the outer epithelial layer of the mouth. Dna carries genetic information that is inherited, or passed down from parents to offspring. Amplification of a specific dna sequence by polymerase.
Used inoculating loopsswabs and slides with cheek cells should be sterilised with. Stepbystep video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells. The alu element found within an intron of the tpa gene is dimorphic. Position a cover slip at a 45 degree angle just inside the left edge of the solution. Please complete your lab report on separate lined paper and include each. Put a drop of water in the center of a glass slide. By the end of the semester you should be familiar with. Extracting human dna in the classroom buccal cheek cells can be harvested painlessly and in sufficient quantity to visualise dna extracted in a simple 4step protocol we will be carrying out an optimised dna. The cells that line the inside of your cheek are called epithelial cells. To observe and identify major animal cell structures and to relate the structure of the cell to its function.
A neat lab sheet introduces cell biologists to the general structure of both animal and plant cells. Place a small amount of onion tissue on the glass slide. Dna is used every day by scientists and lawyers to help in criminal investigation, paternity suits, cloning, etc. Deoxyribonucleic acid dna is a molecule present in all living things, including bacteria, plants, and animals.
In this lab you will look at two types of cells, a human cheek cell and an onion cell and see how they are similar and how they are different. A cell membrane that is semipermeable surrounds the cytoplasm. This dye is toxic when ingested and it causes irritation when in contact with the skin and eyes. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
Pour the mixture into the blender and blend it for no more than 3 seconds. Study 50 terms dna cheek cells lab flashcards quizlet. Alu elements are short interspersed, repeated dna elements distributed through out the primate genomes. Move your fingers down and to the right to place the cover slip over the cheek cell. To view cheek cells, gently scrape the inside lining of your cheek with a toothpick.
Methylene blue stains negatively charged molecules in the cell, including dna and rna. Onion and cheek cell lab microbiology by lillian c. Ocular condenser stage course adjustment knob describe how total magnification is calculated. Fluorescent cell imaging activities for your classroom biorad zoe. If i look at my cheek cell scraping under a microscope and put iodine on it, then i will see the different parts of the cell including the nucleus, cytoplasm, and cell membrane. Onion peel the cells are very clearly visible as compartments with. As in all animal cells, the cells of the human cheek do not possess a cell wall. Buccal cheek cells can be harvested painlessly and in sufficient. This one period lab is a great way to introduce microscope skills or view animal cells using the students own cheek cells. This activity provides an opportunity to accomplish two tasks in our ap biology adventure. They draw and label what they observe under high power. I drank celery juice for 7 days and this is what happened no juicer required. List 2 organelles that were not visible but could be found in cells if you had a microscope with a better magnification.
You can make a lab anywhere by using a cell phone and some household items. Dna extraction lab 1 cheek cell dna extraction capture your genes in a bottle. Human cheek cells experiment from microscopes for schools. The virtual microscope is a little more complicated than the microscope we used in the lab, but it will not be difficult to use. Dna isolation using human cheek cells introduction this experiment shows how to isolate human dna from the cells of your cheek. Take the gatorade from the cup into your mouth, and swish the water around vigorously for 60 seconds. Keeping in mind that the mouth is the first site of chemical digestion in a human.
Turn the microscope nose piece so that the 4x objective is over the light. Ncert class 9 science lab manual slide of onion peel and cheek cells aim to prepare stained temporary mounts of onion peel and human cheek cells and to record observations and draw their labelled diagrams. Take a small drink of water from a nearby drinking fountain and swirl it around in your mouth several times without swallowing and then spit the water into the cup. In this lab, you will prepare and observe cells from both a plant onion and an animal your own cheek.
They are constantly being soughed off like the cells of the outer skin. Today%for%this% laboratory %we%will%be%using%a%compound%light%microscope. You will need to provide slides of cheek cells, onion cells, elodea, and volvox. Read pdf elodea cheek cell lab answers plasmolysis in elodea plant cells science netlinks purpose the purpose of this lab is to learn how to prepare a wet mound, to learn proper staining techniques and to examine human cheek cells and onion skin cells. The main cell structures are easy to see when a stain is used and the slide is viewed with the microscope. Choose one of the cell labs onion cell, cheek cell that you completed this week and report out your findings using the following lab report format. In class, we obtained cheek cells by scraping the inside of the mouth with a toothpick and then rubbing the toothpick on a drop of water with blue. Microscope and the cell lab report purpose of the microscope describe the correct way to handle a microscope. Cells come in a variety of shapes and sizes, and cells perform different functions. Manipulation of apparatus involves manual dexterity and efficiency e. Obtain the cup containing 10 ml gatorade from your instructor.
Students can easily view their own cells as an example of eukaryote cells and cell theory. The light microscope used in the lab is not powerful enough to view other organelles in the cheek cell. In plant and animal cells, similarities and differences exist because of varied life functions. The small blue dots are bacteria from our teeth and mouth. Laboratory exercise dna extraction techniques for use in education received for publication, march 29, 2009 r. Although cells may appear outwardly different, they resemble each other because they share common structures. List 2 organelles that were not visible but should have been in the. Bring the cup containing the water and your cheek cells into the lab and pour a few ml into the larger test tube. Compound microscope describe the location and function of the following parts of the microscope. Cheek cell dna extraction capture your genes in a bottle. This mixes the lysis solution with the cheek cells. Combining two lenses increases the magnification but decreases the. Focus your cell phone microscope on your cheek cells and take a cellfie. The two scales both the micrometer is adjusted so that the zero of two scales merge or.
Virtual microscope lab cheek cells the biology corner. How are cheek cells different from the plant cells you have studied. Laboratory exercise dna extraction techniques for use in. Dna extraction from cheek cells the same general procedures, with little modification, for extracting dna can be used with a variety of plant and animal cells. This procedure teaches the properties of cells, cell. As a result, some of these cells can easily and painlessly be. Using the provided pipet, slowly add 10 ml of the ice cold alcohol by letting it run gently run down the side of the bottle hold the bottle at an angle. Place your thumb over the end of your test tube and. Some scientists have speculated that the evolution of eucaryotic cells involved the merging of two. Observing cells from a human cheek and bacteria under a compound microscope. Cheek cells do not move on their own, so you will not find two organelles that function for cell movement. First of all, we will hopefully be able to see our own dna from.
Add a small drop of iodine stain directly to the tissue. Onion tissue as well as cheek epithelial tissue provide excellent cells to study under the microscope. If you missed the microscope lab we did in class, you will need to make it up by using a virtual microscope which can be accessed on the internet. Introduction to cells, cheek cell and onion cell lab. Unlike plant cells, the cytoplasm in an animal cell is denser, granular and occupies a larger space. Arblaster from the institute of cell and molecular biosciences, university of newcastle upon tyne, uk. This laboratory course is designed to familiarize you with techniques used in cell biology. Learn how to extract dna from cells and describe the purpose of the. The cell lab student report page pre lab questions. In this investigation, you will compare the structures of 2 typical plant cells, onion allium and elodea densa anacharis a common aquarium plant, with a typical animal cell, a cheek cell. Ncert class 9 science lab manual slide of onion peel and. To find out what an animal cells look like under a microscope. Introduction to cells, cheek cell and onion cell lab, lesson powerpoint 1. In this experiment you would use dna isolated from your cheek cells see above as template to amplify a short region of dna within the plasmogen activator gene tpa containing a short 300bp insertion called an alu element.
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